Morphological studies on apoptosis of HCC8204 cell line induced by transferring FasL into HCC9204 cell line

AIM To construct adenovirus vector with FasL, transfer HCC9204 cell line, and observe FasL effects on HCC9204. METHODS Using adenovirus as vector, we cloned FasL gene into vector and identified if FasL gene was cloned into vector by using enzyme; we used Lipofectin to pack vector with FasL, transferred vector into HCC9204 cell line, and then observed morphologic change of HCC9204 cell. RESULTS Enzyme digestion results showed that FasL gene was cloned into adenovirus vector; 72 hours after transferring FasL gene into HCC9204 cell line,FasL gene was expressed at a higher level than that in untransferred cells ( P 0.01); condensation of chromosome and appotosis body were observed under electroscope. CONCLUSION Adenovirus vector with FasL gene is successfully constructed; transferred FasL may induce HCC9204 cell line to display cell appotosis.