Morphological studies on appotosis of GRC-1 cell line induced by transferring FasL into GRC-1 cell line

AIM To construct adenovirus vector with FasL, transfer vector into GRC 1 cell line, and observe FasL effectson GRC 1. METHODS Using adenovirus as vector, cloned FasL gene into vector and identified if FasL gene was cloned into vector by using enzyme digestion; using Lipofectin to pack vector with FasL, transferred vector into GRC 1 cell line, and then observed morphological change of GRC 1 cell. RESULTS ① FasL gene was cloned into adenovirus vector by using enzyme cutting; ② After 72 hours after transferring FasL gene into GRC 1 cell line, FasL gene was higher expression level than untransferred cells; ③ Condensation of chromosome and apoptosis body were observed by electroscope. CONCLUSION Adenovirus Vector with FasL gene was sucessfully constructed; transferring FasL into GRC 1 cell may induced GRC 1 cell line to display cell apoptosis.