2004Food ScienceRequires access

Synthesis and Identification of Immunogen Clenbuterol Residues in Foods

HE Tie-ming

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Abstract

Clenbuterol is a small molecular hapten that has immunoreactivity but hasn't immunogenicity. It is coupled with bovine serum albumin by diazotization in this article. And the Clenbuterol_BSA conjugate is identified by ultra violet scan, SDS- PAGE and indirect inhibited ELISA .The results demonstrated that the Clenbuterol_BSA conjugate has immunogenicity which is good for next monoclonal antibody preparation and the research of ELISA kit .

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What this paper is about

Clenbuterol is a small molecular hapten that has immunoreactivity but hasn't immunogenicity. It is coupled with bovine serum albumin by diazotization in this article. And the Clenbuterol_BSA conjugate is identified by ultra violet scan, SDS- PAGE and indirect inhibited ELISA .The results demonstrated that the Clenbuterol_BSA conjugate has immunogenicity which is good for next monoclonal antibody preparation and the research of ELISA kit .

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Available abstract

Clenbuterol is a small molecular hapten that has immunoreactivity but hasn't immunogenicity. It is coupled with bovine serum albumin by diazotization in this article. And the Clenbuterol_BSA conjugate is identified by ultra violet scan, SDS- PAGE and indirect inhibited ELISA .The results demonstrated that the Clenbuterol_BSA conjugate has immunogenicity which is good for next monoclonal antibody preparation and the research of ELISA kit .

Key concepts: Clenbuterol, Immunogen, Immunogenicity, Conjugate, Hapten, Bovine serum albumin, Chemistry, Monoclonal antibody

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