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Preparation and characterization of a monoclonal antibody specific for the β‐agonist clenbuterol

E. Petruzzelli, A. Ius, Silvia Berta, M. Dovis, Alberto Albertini

Open publisher page 13 citations

Abstract

A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by γ1,κ isotype and an affinity constant of 3.5 × 108 l mol‐1, is highly specific as its cross‐reactivity to structurally related molecules is less than 1%. A competitive ELISA for the detection of clenbuterol in horse and human urine was developed using this antibody.

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What this paper is about

A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by γ1,κ isotype and an affinity constant of 3.5 × 108 l mol‐1, is highly specific as its cross‐reactivity to structurally related molecules is less than 1%. A competitive ELISA for the detection of clenbuterol in horse and human urine was developed using this antibody.

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OpenAlex reports 13 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by γ1,κ isotype and an affinity constant of 3.5 × 108 l mol‐1, is highly specific as its cross‐reactivity to structurally related molecules is less than 1%. A competitive ELISA for the detection of clenbuterol in horse and human urine was developed using this antibody.

Key concepts: Clenbuterol, Monoclonal antibody, Agonist, Chemistry, Antibody, Chromatography, Immunology, Medicine

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