Ampelopsin induces apoptosis of human retinoblastoma HXO-RB_(44) cells

Objective To investigate the effect of ampelopsin on the apoptosis of human retinoblastoma HXO-RB44cells. Methods Human retinoblastoma HXO-RB44cells were treated with different concentration of ampelopsin in vitro for24 h. Cell proliferation was measured by MTT and cell colony formation test. Morphological changes were observed under optical microscopy. Cell apoptosis was observed by AO /EB staining and flow cytometry. Results MTT assay showed that ampelopsin significantly inhibited the growth of cultured HXO-RB44cells with an IC50of 14. 71 mg /L. Cell colony test revealed that the repressive rate was 100. 0% and 52. 2% after treatment with 9. 90 mg /L and 4. 40 mg /L ampelopsin,respectively.AO /EB fluorescent staining showed typical morphological changes of cell apoptosis after the drug treatment,including cell shrinkage,chromatin condensation and nuclear fragmentation. The apoptosis rates were 88. 1%,58. 5%,14. 8% and 4. 8%after 24 h treatment with 50. 00 mg /L,14. 84 mg /L,4. 40 mg /L and 0. 00 mg /L of ampelopsin( P 0. 05). Conclusion Ampelopsin can inhibit the growth and induce the apoptosis of human retinoblastoma HXO-RB44cells in vitro.