The Association of ApoA5 Gene- 1131T > C and 56C > G Polymorphism with Type 2 Diabetes Mellitus in Han People of Guizhou

Objective To study the association of ApoA5 gene-1131T C and 56 C G polymorphism with type2 diabetes mellitus( T2DM) and lipid spectrum in Han People of Guizhou. Methods 192 T2DM unrelated patients received by the Affiliated Hospital of Guiyang Medical College during the period from March 2012 to March 2013 were selected as the T2DM group while 206 people with normal level of blood lipid and blood sugar once taking physical examination in our hospital during the same period as the control group. In order to examine the genotype of ApoA5- 1131T C and 56C G,the technology of agarose gel-electrophoresis combined with polymerase chain reaction / restriction fragment length polymorphism( PCR- RFLP) was adopted. The frequency distribution of the allelic genes was also recorded while the level of blood lipid and blood sugar were examined with automatic chemistry analyzer. Results The distribution of genotype of ApoA5- 1131T C in the control group( χ2= 0. 477,P = 0. 489) and T2DM group( χ2= 1. 597,P = 0. 206) has been found to conform to the Hardy- Weinberg law of genetic equilibrium and was also representative of the group. The genotype and allele frequency of the two groups showed statistically significant difference( P 0. 05). The 1131C type carriers( TC + CC) had an 1. 965 times increased risk of T2DM compared with TT genotype( OR = 1. 965) and had a significant increase in TG levels compared with non- C carriers( TT)( P 0. 05). ApoA5 56C G was in exon2 of ApoA5,and only CC genotype was detected. Conclusion ApoA5- 1131T C polymorphism is associated with the risk of T2DM in Han People of Guizhou. C allele has a significantly increased T2DM risk and is associated with the increased serum TG levels. We do not observe ApoA5 gene 56C G polymorphism.