Study of apolipoprotein A5 gene polymorphism in the aged patient with essential hypertension in Han people in Qingdao region

Objective To investigate the association between-1131T/C polymorphism in apolipoprotein A5(APOA5)gene and the aged patient with essential hypertension in Han people in Qingdao region.Methods The genotypes and allele frequency distribution of APOA5-1131T/C gene polymorphism in 130 aged patients with essential hypertension(aged hypertensive group) and 120 cases of healthy persons(control group) were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method.The body mass index(BMI),level of blood pressure and life style were collected in two groups,and the levels of blood fat,lipoprotein and blood sugar were detected by method of biochemistry.Results Smoking history,family history of hypertension,the levels of BMI,systolic blood pressure(SBP),diastole blood pressure(DBP),total cholesterol(TC),triglycercide(TG) and low density lipoprotein-cholesterol(LDL-C) in aged hypertensive group were higher than those in control group,the level of high density lipoprotein-cholesterol(HDL-C) was lower than that in control group(P0.01).PCR amplified fragment of APOA5-1131T/C gene polymorphism was 188 bp.The genotypes distribution of APOA5-1131T/C gene polymorphism were TC-type locus in common,followed by TT-type,and CC-type was relatively rare in aged hypertensive group,and TT-type locus in common,followed by TC-type,and CC-type was relatively rare in control group.T allele was a common allele and C allele was a rare allele in the allele frequency distribution in two groups.There were statistically significant differences in genotype and allele frequency distribution in two groups(P0.05,P0.01).Relative risk analysis of APOA5 genotype frequency showed the risk of suffer from hypertension for CC-type homozygous and TC-type heterozygous higher than that in TT homozygous(P0.05).The levels of TG in TC-type and CC-type of APOA5-1131T/C gene polymorphism were higher than those in TT-type(P0.01).There was no statistically differences in other lipid parameters among the genotypes(P0.05).Conclusion The APOA5-1131T/C gene polymorphism might affect the level of plasma TG,and APOA5-1131C allele may be independent risk factor for the aged patient with essential hypertension in Han people in Qingdao region.