Detection Method of the Aerolysin Gene in Pathogenic Aeromonas hydrophila Based on Polymerase Chain Reaction

[Objective] To establish a novel detection method of the Aerolysin gene in pathogenic Aeromonas hydrophila based on polymerase chain reaction(PCR).[Method] The Aeromonas hydrophila were isolated from the infection focus of crucian with plate streaking.DNA was extracted by CTAB method.P1 and P2 primers were designed according to the conserved sequence of pathogenic Aeromonas hydrophila aerolysin gene registered on GenBank.The comparative analysis of sequencing results was performed by Blast method.[Result] DNA fragment of Aeromonas hydrophila was about 540 bp.Blast analysis showed that the homology between amplified gene and aerolysin gene logged GenBank was up to 95.67%.[Conclusion] The method is simple and feasible for detecting aerolysin gene in pathogenic Aeromonas hydrophila.