Study on the HPLC Fingerprints of Fructus Gardeniae Established with Dual-wavelength Fusing Method

Objective To explore the HPLC-FPS of water extract of Fructus Gardeniae by dual-wavelength fusing method.MethodsThe chromatographic fingerprints were obtained by injecting the sample solution each time on a CenturySIL C18 BDS column(20 cm×4.6 mm,5 μm)with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acid-acetonitrile.The flow rate was 1.0ml/min,the column temperature was maintained at(30.00±0.15)℃ and the DAD detector was used.ResultsThe HPLC-FPS of water extract of Fructus Gardeniae was established with dual-wavelength fusing method,acquired 41 common peaks by taking the peak of geniposide as referential peak.The fingerprints were comprehensively evaluated by The digitized evaluation system of the Traditional Chinese Medicine fingerprint with the super-information and the results were analyzed and compared with that of the fingerprints obtained under 265 nm.ConclusionThe dual-wavelength fusing method can effectively control the quality of Fructus Gardeniae and overcome the low information of mono-wavelength detection.It offers guarantee for the quality control of Fructus Gardeniae and provides a new method for thoroughly and authentically revealing the characteristics of TCM.