Multi-index evaluation on the optimization of extraction process in Fructus Gardeniae from Bazhong

OBJECTIVE To inspect the extraction process of Fructus Gardeniae from Bazhong by multi-index evaluation.METHODS To study the factors that play an important role on the active ingredients of Fructus Gardeniae by orthogonal design,such as material-liquid ratio,extraction times,extraction time and ultrasonic frequency.With HPLC along with ELSD to detect the content of geniposide,crocin I,ursolic acid.RESULTS The outcome confirmed that the yield of main active ingredients in Fructus Gardeniae extracted by water was high,the best extraction parameters of geniposide were as follows: material-liquid 1∶15,extracted 3 times,extracting 15 min,with the ultrasonic frequency of 100 kHz;and the extraction method of ursolic acid was to select ethanol as the solvent to draw the Fructus Gardeniae dregs extracted by water,the best extraction indexes were as follows: material-liquid 1∶10,extracted 2 times,extracting 60 min,with the ultrasonic frequency of 100 kHz.Combined with statistical software,established multiple nonlinear regression equation between Z-score and extraction times,extraction time,frequency ultrasonic,got the following equation:Y=2.871 864X12+0.002 283X22-0.824 94X32+0.035 945X1X2+0.702 867X1X3-0.126 25X2X3-13.271 6X1+0.017 6X2+8.144 655X3,R=0.999 9.CONCLUSION Multi-index evaluation on the extraction process of Fructus Gardeniae from Bazhong is more comprehensive and objective.Validation experiments confirmed that the process is stable,worthy of popularizing.