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Reconstruction and expression of ORF2 Gene of Porcine Circovirus Type 1 in E.coli

Changxu Song

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Abstract

A pair of primers was designed according to PCV1 ORF2 genome sequence in the GenBank. The ORF2 genome was cloned into pMD 18-T vector, and the recombinant plasmid was named pMD-ORF2. Then the ORF2 genome was subcloned into prokaryotic expressing vector pET-32α and pET-41α,the recombinant plasmid were named pET-ORF2-1 and pET-ORF2-2 respectively, the recombinant plasmid was induced by IPTG. The results of SDS-PAGE and Western-blot indicated that the ORF2 gene was expressed in high level, and the recombinant fusion protein was about 30Kda and 45 Kda.

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What this paper is about

A pair of primers was designed according to PCV1 ORF2 genome sequence in the GenBank. The ORF2 genome was cloned into pMD 18-T vector, and the recombinant plasmid was named pMD-ORF2. Then the ORF2 genome was subcloned into prokaryotic expressing vector pET-32α and pET-41α,the recombinant plasmid were named pET-ORF2-1 and pET-ORF2-2 respectively, the recombinant plasmid was induced by IPTG. The results of SDS-PAGE and Western-blot indicated that the ORF2 gene was expressed in high level, and the recombinant fusion protein was about 30Kda and 45 Kda.

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Available abstract

A pair of primers was designed according to PCV1 ORF2 genome sequence in the GenBank. The ORF2 genome was cloned into pMD 18-T vector, and the recombinant plasmid was named pMD-ORF2. Then the ORF2 genome was subcloned into prokaryotic expressing vector pET-32α and pET-41α,the recombinant plasmid were named pET-ORF2-1 and pET-ORF2-2 respectively, the recombinant plasmid was induced by IPTG. The results of SDS-PAGE and Western-blot indicated that the ORF2 gene was expressed in high level, and the recombinant fusion protein was about 30Kda and 45 Kda.

Key concepts: Recombinant DNA, Porcine circovirus, Plasmid, GenBank, Biology, Molecular biology, Genome, Gene

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Reconstruction and expression of ORF2 Gene of Porcine Circovirus Type 1 in E.coli — Research Paper | ScholarLens