Cloning and Prokaryotic Expression of ORF1 Gene of Porcine Circovirus Type 2

This study was conducted to design,a pair of primer according to PCV2 ORF1 gene sequence in the Genbank.The ORF1 gene was amplified from suspected PMWS sample via polymerase chain reaction(PCR),and the gene was cloned into pMD18-T vector.Then the ORF1 gene was subcloned into prokaryotic expressing vector pET-32a,the recombinant plasmid was named pET-32a-ORF1,and was induced by IPTG.The results of SDS-PAGE and Western-blotting indicated that the ORF1 gene was expressed in a high level,and the recombinant fusion protein was about 54 kDa.The recombinant fusion protein has peculiar reaction to PCV2 positive serum which has immunological activity.