Relationship between polymorphism of the human intestinal fatty acid binding protein gene and type 2 diabetic patients with coronary heart disease

Objective To study the relationship between the human intestinal fatty acid binding protein gene (FABP2) and type 2 diabetic patients with coronary heart disease. Methods The polymorphism of 54th codon of FABP2 was determined with the PCR and RFLP methods in 268 subjects, inclnding 82 cases of type 2 diabetes with coronary heart disease (CHD), 64 cases of type 2 diabetes without CHD, 62 cases of non diabetic CHD and 60 normal controls. Results The polymorphic restriction site has been reported in the FABP2 identified by the enzyme Hha I in Han nationality in Wuhan. The frequency of genotype Thr54(+) significantly increased in the type 2 diabetic patients with coronary heart disease as compared with that in healthy subjects (P0.05). There was no significant difference in the frequencies among coronary heart disease, type 2 diabetes, type 2 diabetic patients with coronary heart disease and the control groups, either in genotypes or in alleles. The attributable risk of FABP2 Thr54(+) genotype for the type 2 diabetic patients with coronary heart disease was 25.6%, and odds ratio was 2.13 (95% confidence interval is 1.05～4.45). The patients of Thr54(+) genotype with coronary heart disease had significantly higher fasting insulin level and plasma triglyceride value than those of Thr54(-) genotypepatientswithcoronaryheart disease (P0.01,P0.05). The patients of Thr54(+) genotype had significantly lower insulin sensitive index and lower high density lipoprotein cholesterol (HDL C) than those of Thr54(-) genotype in type 2 diabetic patients with coronary heart disease (P0.05). Conclusions FABP2 gene polymorphism seems to be associated with the type 2 diabetic patients with coronary heart disease and their insulin resistance. FABP2 gene polymorphism also may be involved in the determination of lipoprotein profiles in the type 2 diabetic patients with coronary heart disease.