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Study on removal of protein from lentinan

Long Haiyan

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Abstract

In a condition of alkalescent solution,polysaccharide solution which has been extracted with heating water leaching from lentinus ododes was treated with CAS,enzyme-decomposing dialysis and active carbon to remove protein,then flocculated with organic mixed solvent to reclaim polysaccharide and frozen-dry to remove moisture.After been determined,protein and glucuronic acid have been removed cleanly,recovery of polysaccharide was 0.67%,color of sample was white.The results demonstrated that this method can efficiently remove protein,pectin,nucleic acid and so on.

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What this paper is about

In a condition of alkalescent solution,polysaccharide solution which has been extracted with heating water leaching from lentinus ododes was treated with CAS,enzyme-decomposing dialysis and active carbon to remove protein,then flocculated with organic mixed solvent to reclaim polysaccharide and frozen-dry to remove moisture.After been determined,protein and glucuronic acid have been removed cleanly,recovery of polysaccharide was 0.67%,color of sample was white.The results demonstrated that this method can efficiently remove protein,pectin,nucleic acid and so on.

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Available abstract

In a condition of alkalescent solution,polysaccharide solution which has been extracted with heating water leaching from lentinus ododes was treated with CAS,enzyme-decomposing dialysis and active carbon to remove protein,then flocculated with organic mixed solvent to reclaim polysaccharide and frozen-dry to remove moisture.After been determined,protein and glucuronic acid have been removed cleanly,recovery of polysaccharide was 0.67%,color of sample was white.The results demonstrated that this method can efficiently remove protein,pectin,nucleic acid and so on.

Key concepts: Chemistry, Polysaccharide, Lentinan, Pectin, Chromatography, Nucleic acid, Leaching (pedology), Moisture

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