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2011•Unpublished venueRequires access

Detection of genes expression for CD44 and CD24 on dendritic cells before and after stimulation by lipopolysaccharide

Wenchao Liu

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Abstract

Objective:To detect the genes expression for CD44 and CD24 on dendritic cells(DC) before and after stimulation by lipopolysaccharide(LPS).Methods:Human monocytes were isolated from heparinized whole blood and then induced into DC by adding rhGM-CSF and rhIL-4 for 6d.After that,DCs were induced with or without LPS for 24h and total cellular RNA were extracted for real time PCR analysis.Results:Expressions of CD44 and CD24 were lower in monocytes-derived DC induced by rhGM-CSF and rhIL-4 for 6d.After being stimulated by LPS for 24h,the expression of CD44 and CD24 increased significantly(P0.05).Conclusion:The expression of CD44 and CD24 on DC increased after being stimulated by LPS.This is related to functional maturation of DC.

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What this paper is about

Objective:To detect the genes expression for CD44 and CD24 on dendritic cells(DC) before and after stimulation by lipopolysaccharide(LPS).Methods:Human monocytes were isolated from heparinized whole blood and then induced into DC by adding rhGM-CSF and rhIL-4 for 6d.After that,DCs were induced with or without LPS for 24h and total cellular RNA were extracted for real time PCR analysis.Results:Expressions of CD44 and CD24 were lower in monocytes-derived DC induced by rhGM-CSF and rhIL-4 for 6d.After being stimulated by LPS for 24h,the expression of CD44 and CD24 increased significantly(P0.05).Conclusion:The expression of CD44 and CD24 on DC increased after being stimulated by LPS.This is related to functional maturation of DC.

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Available abstract

Objective:To detect the genes expression for CD44 and CD24 on dendritic cells(DC) before and after stimulation by lipopolysaccharide(LPS).Methods:Human monocytes were isolated from heparinized whole blood and then induced into DC by adding rhGM-CSF and rhIL-4 for 6d.After that,DCs were induced with or without LPS for 24h and total cellular RNA were extracted for real time PCR analysis.Results:Expressions of CD44 and CD24 were lower in monocytes-derived DC induced by rhGM-CSF and rhIL-4 for 6d.After being stimulated by LPS for 24h,the expression of CD44 and CD24 increased significantly(P0.05).Conclusion:The expression of CD44 and CD24 on DC increased after being stimulated by LPS.This is related to functional maturation of DC.

Key concepts: CD24, Lipopolysaccharide, CD44, Stimulation, Molecular biology, Gene, Flow cytometry, Gene expression

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