2010•Xiandai shengwu yixue jinzhanRequires access
Culture of dendritic cells in adult peripheral blood in vitro.
Ying Wang, Wu DePing, Yang FeiLan, Guan Min, Lirong Zhang, Ya Wang, Li SanQing, Jia‐Lin Wang
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Abstract
Objective:To analyse the mature dynamic and phenotype molecular changes of human peripheral blood-derived den-dritic cells(DCs) in vitro;Methods:Monocytes,which were isolated from human peripheral blood mononuclear cells,were cultured in medium containing IL-4 and GM-CSF.After 5～6 days of culture to detects the mature marker CD83 and co-stimulatory molecules:CD58,CD54,CD80,CD86,CD40,HLA-DR,HLA-ABC,which was determined by fluorescence activated cell sorter(FACS);Results:After 5～6 days of culture,more than 95% of cells converted into iDCs with phenotype of CD11c+,CD54low,CD83low,CD40low,CD58low,CD86low,and CD80low,which was determined by fluorescence activated cell sorter(FACS).E.coli LPS-pulsed DCs exhibit-ed significantly high expression of mature marker CD83 and co-stimulatory molecules:CD58,CD54,CD80,CD86,CD40.Conclusions:These results suggest that human monocyte-derived DCs are immature after 5～6 days of culture.