Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk.of Garlic
Linqing Zhang
Abstract
Linqing Zhang
Abstract
A test of crude toxin toxicity using garlic root inhibition was examined from toxin production related to culture medium,temperature,pH,culturing duration,light,dark or shaking conditions.Investigations concerned a garlic white rot pathogen,Sclerotium cepivorum Berk.Results showed that optimum conditions included using a Fries liquid medium at pH 5.0 and at 18.0 ℃,with successive dark periods and shaking during culturing.Production of crude toxin reached the highest level on the 6th day of culturing.
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A test of crude toxin toxicity using garlic root inhibition was examined from toxin production related to culture medium,temperature,pH,culturing duration,light,dark or shaking conditions.Investigations concerned a garlic white rot pathogen,Sclerotium cepivorum Berk.Results showed that optimum conditions included using a Fries liquid medium at pH 5.0 and at 18.0 ℃,with successive dark periods and shaking during culturing.Production of crude toxin reached the highest level on the 6th day of culturing.
Key concepts: Sclerotium, Toxin, Pathogen, White rot, Liquid medium, Biology, Toxicity, Horticulture