Xiaomin Mixture on Chronic Allergic Contact Dermatitis in Mice Serum IFN-γ,IL-4 Influence of Content Randomized Controlled Study

[Objective] To observe the effect of eliminating sensitive mixture on chronic allergic contact dermatitis in mice serum ICAM-1, NF- κ B content.[Method] Using randomized controlled methods, 50 healthy Kunming mice were randomly marked number were divided into 5 groups, 10 rats in each group(blank control group, model group, Xiaomin mixture group, loratadine group, Xiaofengzhiyang granules group). The use of 2,4- two nitro chlorobenzene(DNCB) induced murine allergic contact dermatitis(ACD) model. Before modeling 1 day stomach intervention, 0.4mL/ times, 1 times /d, until the last stimulate after 6h; blank control group, ACD model group, normal saline; the other groups by body surface area reduced, according to every adult equivalent dosage concentration 4 times respectively Xiaomin mixture, loratadine, Xiaofengzhiyang granules stomach intervention. The ELISA method for the determination of serum ICAM-1, NF-κ B level. [Results] The peripheral white blood cell count Xiaomin mixture group, loratadine group, xiaofengzhiyang granules group were significantly decreased(P 0.01), the model group was significantly higher than that in the control group(P 0.01), xiaofengzhiyang granules group and anti sensitive mixture was higher than that of loratadine group(P 0.01). Lesions of ICAM-1, NF- κ B content in each group were significantly higher than that in the control group(P 0.01), the intervention group were significantly lower than those in the model group(P 0.01), anti sensitive mixture group was significantly lower than that of xiaofengzhiyang granules group(P 0.01), xiaofengzhiyang granules group was higher than that of loratadine group(P0.01). The expression of ICAM-1 in blank control group the positive expression was not obvious within the epidermis, dermis cells only wrapped slurry with a small amount of or scattered in the brown granules, staining,model group, positive expression in epidermis, epidermic cells, cytoplasm containing large amounts of brown granules, darker, Xiao Minhe agent group, loratadine group and the consumer the wind antipruritic pellet group showed positive brown staining granules in different degree, but compared with the model group, the expression quantity are few, scattered in the cytoplasm brown granules. The expression of NF- κ B blank control group,positive expression was not obvious in the epidermis, dermis cells only wrapped slurry with a small amount of or powder granules stained in brown, stained lighter; model group was expressed in epidermis, epidermic cells,cytoplasm containing large amounts of brown granules, darker; Xiao Min Decoction group, loratadine group and xiaofengzhiyang granules group showed different degree of positive brown granules, compared with the model group, the expression quantity are few, scattered in the cytoplasm brown granules. [Conclusion] Anti sensitive mixture can regulate the expression of NF- κ ICAM-1, B cytokines, on DNCB induced chronic allergic contact dermatitis inflammatory response inhibi.