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The Optimal Fermentation Condition of Endophytic Bacteria B_(36) Isolated from Sophora flavescens Ait with Antifungal Activity

Yang Zhang

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Abstract

The culture conditions and the compositions of cultural medium of Endophytic B36 strain isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method.The optimal fermentation parameters were 28℃,150r·min-1,pH 9.0,20% inoculation volume,and 50mL culture in 250mL flask for 36h,which had the strongest antagonistic activity.The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%,yeast 0.5%,soybean flour 0.5%,NH4NO30.5% and CaCO31.0%.

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What this paper is about

The culture conditions and the compositions of cultural medium of Endophytic B36 strain isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method.The optimal fermentation parameters were 28℃,150r·min-1,pH 9.0,20% inoculation volume,and 50mL culture in 250mL flask for 36h,which had the strongest antagonistic activity.The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%,yeast 0.5%,soybean flour 0.5%,NH4NO30.5% and CaCO31.0%.

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Available abstract

The culture conditions and the compositions of cultural medium of Endophytic B36 strain isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method.The optimal fermentation parameters were 28℃,150r·min-1,pH 9.0,20% inoculation volume,and 50mL culture in 250mL flask for 36h,which had the strongest antagonistic activity.The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%,yeast 0.5%,soybean flour 0.5%,NH4NO30.5% and CaCO31.0%.

Key concepts: Sophora flavescens, Fermentation, Sophora, Sucrose, Yeast extract, Food science, Antifungal, Yeast

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