Stereological study on the effect of Nbn gene on the development of hippocampal dentate gyrus in mice

Objective To investigate the role of Nbn gene in the development of hippocampal dentate gyrus(HDG) in Nbn gene neuro-specific deleted(Nbn-CNS-del) mice by histopathology.Methods NeuN monocloned antibody,immunohistochemistry and stereological methods were used to quantitatively analyze the morphological changes of HDG in Nbn-CNS-del mice and Nbn gene undeleted mice(control groups) on P7,14 and 21st day.Results Comparing with the control group,the profile areas of each layers in HDG of postnatal Nbn-CNS-del mice were all cut down(P0.05),and the profile numberical densities of NeuN positive cells in granular cell layer were also decreased(P0.05).Additionally,the profile numberical densities of NeuN positive cells in polymorphic layer were still higher than that of control group(P0.05).Conclusion The development of HDG in Nbn gene neuro-specific deleted mice is inhibited than that in control mice.Nbn gene might be an important gene in the development of mouse hippocampal dentate gyrus.