Construction and in vivo Expression of Trichinella spralis T626-55 Gene Vaccine Carried by Attenuated Salmonella

Highly immunogenic gene T626-55 of Trichinella spiralis was amplified using PCR reaction and cloned into pcDNA3.1 eukaryotic expression plasmid.The recombinant plasmid was transformed into S.enterica Typhimurium phoP/phoQ strain by electroporation.In vivo expression of the target gene was testified.Mice were orally administrated with the recombinant bacteria and after 5 days of administration,spleen and Peyer′s patch were aseptically removed for reverse transcription-polymerase chain reaction(RT-PCR) and indirect fluorescence assay(IFA).The results show that Salmonella harboring T626-55 gene coding eukaryotic plasmid T626-55-pcDNA3.1-PQ was successfully constructed.The transcription of T626-55 gene was detected in spleen and pp,and expression of T626-55 protein was confirmed in spleen.