2004•PubMedRequires access
[Construction of DNA vaccine of Trichinella spiralis muscle larvae and its expression in mice].
Jing Cui, Hongwei Zhang, Zhongquan Wang, Yonglong Li
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Abstract
OBJECTIVE: To construct and express recombinant plasmid containing the structural gene encoding Mr 31 000 antigen of Trichinella spiralis muscle larvae. METHODS: The target gene TspE1 was amplified by RT-PCR, cloned into pUC18 vector, and sub-cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3-TspE1 by gene-gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining. RESULTS AND CONCLUSION: The recombinant plasmid pcDNA3-TspE1 was successfully constructed and expressed in the BALB/c mice.