Effects of Chuanxikang decoction on the amount of IFN-γ and IL-5 in bronchoalveolar lavage fluid and expression of MMP-9 and TIMP-1 in lung tissue of asthmatic model in guinea pig

Objective:To investigate the effects of Chuanxikang decoction(a Chinese herbal medicine)on the amount of IFN-γ and IL-5 in bronchoalveolar lavage fluid(BALF) and expression of MMP-9 and TIMP-1 in lung tissue of asthmatic model in guinea pig to explore its mechanism in treatment of bronchial asthma.Methods:A total of 40 guinea pigs were randomly divided into 4 groups:normal control group(A group),asthmatic model group(B group),treatment with dexamethasone(C group) or with Chuanxikang decoction groups(D group),10 guinea pigs in each group.The guinea pig models of asthma were established through injecting ovalbumin(OVA) into the abdominal cavity and inhalation of atomization.The guinea pigs of asthma underwent with different therapy.Amount of IFN-γ and IL-5 in BALF and expression of MMP-9 and TIMP-1 in lung tissue were measured by immunotisssuchemical technology.Results:Compared with the normal control group(A group),the concentrations of IFN-γ in the BALF was decreased significantly(P0.O1)and IL-5 was increased significantly(P0.01) in the asthmatic model group(B group),the content and the relative value MMP-9 and TIMP-1 in lung tissues were also increased obviously(P0.05-0.01);IFN-γ in the BALF of the dexamethasone group(C group) and Chuanxikang decoction groups(D group)was increased significantly(P0.01) and IL-5 was decreased significantly(P0.01),the content and the relative value MMP-9 and TIMP-1 in lung tissues also were decreased obviously(P0.05-0.01) as compared with that in asthmatic model group(B group).Conclusion:Chuanxikang has therapeutic effect on bronchial asthma by increasing the level of IFN-γ and inhibiting the expression of IL-5 in the BALF;by decreasing the the content and the relative value of MMP-9 and TIMP-1 in lung tissue,which may lead to restrain airway remolding.