Simultaneous Quantitative Analysis of Polygonin,Resveratrol, Archen and Emodin Monomethyl Ether in Polygoni Cuspidati Rhizoma et Radix by Multi-Components Assay and Single Marker

Objective:To establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four components in Polygoni Cuspidati Rhizoma et Radix.Method: A HPLC method was developed as QAMS to determine polygonin,resveratrol,archen and emodin monomethyl ether in Polygoni Cuspidati Rhizoma et Radix.The relative correction factor(RCF) of other three components was determined by HPLC using archen as the internal reference substance.Content of the four components in 11 batches of samples,collected from different areas,was determined by both external standard method and QAMS.The method was evaluated by comparison of the quantitative results between external standard method and QAMS.Result: RSD of QAMS method and external standard method was less than 3%,which indicated that no siginificant differences between the quantitative results of QAMS method and external standard method were observed.Conclusion: It is feasible and suitable to evaluate the quality of Polygoni Cuspidati Rhizoma et Radix by QAMS.