Young Leaf Tissue Cultures and Efficient Plant Regeneration in Peanut

Somatic embryogenesis and plant regeneration in young leaf tissue cultures of peanut (Arachis hypogaea L.) cultivar BaiShaGuo were studied. The young leaves were cut into 1 mm×1 mm, and cultured on MS medium supplemented with 1～3 mg/L 2,4-D and 1～5 mg/L BAP. High frequency (89% to 96%) embryogenic clusters derived from leaf explant were obtained on all media used. When the embryogenic clusters were being transferred onto MS medium supplemented with 10 mg/L BAP, or supplemented with 3 mg/LBAP and 1 mg/L ABA, 57% to 100% of calli with embryogenic cluster formed somatic embryos. The emberyos germinated and developed into shoots after being transferred onto MS medium supplemented with BAP (3, 4, 10 mg/L) and ABA (0, 1 mg/L). The highest frenquency of shoot regeneration was up to 96%.