Efficient plant regeneration by tissue cultures in peanut

Efficient adventitious shoot formation and plant regeneration by tissue cultures in peanut(Arachis Hypogaea L.) was studied using 14 cultivars.The immature leaf,cotyledon and hypocotyl explants were cultured on MSB5 induction medium(MS salts and B5 vitamins) supplemented with 0～2 mg/L NAA and 0～10 mg/L BAP.Then the formed calli were transferred onto differentiation medium containing 0～10 mg/L BAP for shoot induction.The result showed:① the concentration of NAA and BAP added to medium successfully influenced the shoot regeneration frequencies,the suitable induction medium was MSB5 medium added with 1 mg/L NAA and 6 mg/L BAP,and the suitable differentiation medium was MSB5 medium supplemented with 4 mg/L BAP;② the immature leaf had higher frequency of shoot formation than cotyledon and hypocotyl;③ the frequencies of shoot formation were different due to the difference in cultivars,the higher frequencies were obtained in cultivar Baisha 1016(91.8%) and Huayu 23(88.5%).The regenerated plants flowered and set pod normally after transplanted in pots.