Optimization of fermentation process for enramycin

In order to improve enramycin production,both fermentation medium and fermentation conditions in flask were optimized.The optimum culture determined was as follows:glucose 2.0,soluble starch 4.0,corn steeping liquor 1.0,corn gluten meal 3.0,cold pressed soybean flour 1.5,ammonium chloride 0.5,magnesium sulfate 0.01,isoleucine 0.2,sodium chloride 1.5,calcium carbonate 2.0.The optimum fermentation conditions were ascertained;culture temperature 28 ℃,rotation speed 225 r/min,pH 7.0 before sterilization,80 mL medium in 750 mL flask,fermentation time 8.5 d.The last fermentation titer was improved approximately 6 times than that of the original.