Optimization of Preparation Technology of Acetic Acid by Circulating Spraying Fermentation

Acetic acid bacteria(Acetobacter sp.) was used to ferment vinegar.The fermentation rate and vinegar yield of static fermentation,shake flask fermentation and circulation spraying fermentation were compared.And the fermentation conditions of circulation spraying fermentation were optimized by single factor experiments and orthogonal test.The results showed that compared with static fermentation and shake flask fermentation,circulation spraying fermentation could shorten the fermentation time while increase the fermentation yield.The optimum circulation spraying fermentation conditions were using corn cob as fermentation bed carrier;fermentation time,24 h;fermentation temperature,32 ℃;pumping capacity,2.7 L / min;inoculum concentration,75 g / L;volume fraction of ethanol,8%.Yield of vinegar could be up to 1.93 g / 100 mL under these conditions.