The experimention of opposing natural recovery of liver fibrosis model in rats

Obejctive For a viable method to oppose the natural recovery trend of CCL4-induced liver fibrosis model in rats by intraperitoneal injection after stopping induction.To ensure that drugs,cell transplantation and other methods of treatment to liver fibrosis isreliable.Methods 50%CCL4 intraperitoneal injection,twice a week,sustaining six weeks induced liver fibrosis model in rats,Then all rats are divided into 4 groups randomly and subcutaneous injected with different doses 50% CCL4(0.02～0.08ml/100g weight),once a week,continuing four weeks,through the liver index,fibrosis stage,fibrosis semi-quantitative statistical analysis,liver function and serum index of liver fibrosis to assess liver fibrosis.Results CCL4 intraperitoneal injection six weeks,the header district of liver tissue and blood vessels appear coarse fibers interval.In anti-reversal experimentation's first two weeks and four weeks,the statistical difference of the level between fibrosis model and subcutaneous injection of 0.06ml/100g weight CCL4 group was not significant(P0.05).Conclusion To a successful rat liver fibrosis model,stopping intraperitoned and altering subcutaneous injection of 50% CCL4 0.06ml/100g weight,once a week,could be able to maintain the level of fibrosis four weeks.