Cryopreservation of the Chicken PGCs Using Different Freezing Media and Equilibrium Methods

Primordial germ cells (PGCs) were isolated from gonads of development stage 19 and 28 by Ficoll density gradient centrifugation. The PGCs were frozen by different cryopreservation media and equilibrium steps. Thawed PGCs were cultured in vitro in DMEM medium, and the vitality of the PGCs were identified by the Trypan Blue exclusion method. The main results showed below: ① Vitality of the PGCs from stage 19 and 28 which were frozen in a same cryopreservation medium showed significant difference or among the different equilibrium steps. Cryopreservation effects of the PGCs frozen by the step 1 were better than those by the step 2. ② Among different cryopreservation media by the same step, media C showed more effective, followed by medium E and F while medium A, B and D was less effective. Vitality time of PGCs showed no significant differences between the frozen-thawed treatment and the control treatment during culture of the tested PGCs in vitro.