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Effect of different cryoprotectants on the cryopreservation of chicken EPGCs

Guohong Chen

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Abstract

This paper focused on isolated primordial germ cells(PGCs) from gonads at stage 19 and stage 28 by Ficoll density-gradient centrifugation.Then,the PGCs were frozen in five kinds of freezing media under the freezing process.The viability of the frozen-thawed PGCs was measured by the trypan blue exclusion method.The results showed:①For the stage 19,the viability of the frozen-thawed PGCs was significant difference(P0.05) between freezing media Ⅰ(10% DMSO+10% FBS) and freezing media Ⅳ(10% EG+10% FBS+0.1 mol/L sucrose),but that of the stage 28 showed no significant difference(P0.05) among all the freezing media.②When cultured on feeder cells in the presence of the three kinds of cytokines,the third passage PGCs still can proliferate and maintain its undifferentiated state.

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What this paper is about

This paper focused on isolated primordial germ cells(PGCs) from gonads at stage 19 and stage 28 by Ficoll density-gradient centrifugation.Then,the PGCs were frozen in five kinds of freezing media under the freezing process.The viability of the frozen-thawed PGCs was measured by the trypan blue exclusion method.The results showed:①For the stage 19,the viability of the frozen-thawed PGCs was significant difference(P0.05) between freezing media Ⅰ(10% DMSO+10% FBS) and freezing media Ⅳ(10% EG+10% FBS+0.1 mol/L sucrose),but that of the stage 28 showed no significant difference(P0.05) among all the freezing media.②When cultured on feeder cells in the presence of the three kinds of cytokines,the third passage PGCs still can proliferate and maintain its undifferentiated state.

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Available abstract

This paper focused on isolated primordial germ cells(PGCs) from gonads at stage 19 and stage 28 by Ficoll density-gradient centrifugation.Then,the PGCs were frozen in five kinds of freezing media under the freezing process.The viability of the frozen-thawed PGCs was measured by the trypan blue exclusion method.The results showed:①For the stage 19,the viability of the frozen-thawed PGCs was significant difference(P0.05) between freezing media Ⅰ(10% DMSO+10% FBS) and freezing media Ⅳ(10% EG+10% FBS+0.1 mol/L sucrose),but that of the stage 28 showed no significant difference(P0.05) among all the freezing media.②When cultured on feeder cells in the presence of the three kinds of cytokines,the third passage PGCs still can proliferate and maintain its undifferentiated state.

Key concepts: Cryoprotectant, Cryopreservation, Ficoll, Trypan blue, Biology, Andrology, Centrifugation, Germ

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Effect of different cryoprotectants on the cryopreservation of chicken EPGCs — Research Paper | ScholarLens