The study of relationship between expression of MMP-9 and microvessel density in astrocytoma

Objective:To investigate the expression of MMP-9 in different kinds of astrocytoma,and their correlation with microvessel density(MVD),and relation with malignancy degree of astrocytoma.Methods:Immmunohistochemical staining was performed to detect the expression of MMP-9 and CD34 in astrocytoma of 50 cases.Expression of CD34 was used to estimate MVD.Results:Expression of MMP-9 protain were detected in all cases in astrocytoma.Positive rates were different in various astrocytomas.There are significant difference between the various groups(P0.01).Positive rates of MMP-9 was(76.52±3.38)% and MVD was(87.00±4.18)/HPF in WHO Ⅲ~Ⅳ grade astrocytomas.In WHO Ⅰ~Ⅱ grade,the expression levels were(27.90±2.53)% and MVD was(48.62±4.05)/HPF.The positive rates of expression of MMP-9 in WHO Ⅲ~Ⅳ grade was higher than in WHO Ⅰ~Ⅱ grade(P0.01);The expressions of MVD,MMP-9 present positive correlation in tissues of astrocytoma.Conclusion:It suggested that expression levels of MMP-9 and MVD were associaed with histological types and malignancy degree and may be markers to judge malignancy degree and prognosis in astrocytomas.