Expression and significance of HIF-1α in Human Meningioma

Objective To study the expression of hypoxia-inducible factor 1(HIF-lα) and the microvessel density (MVD) in meningioma and the relationship between HIF-lα and the grade.To investigate the value of HIF-lα in clinical treatment of meningioma.Methods 45 archival paraffin embedded tissue blocks of meningioma with original clinical and pathological records and 10 normal brain tissue were included in this study.The expression of HIF-lα was immunohistochemically studied in 45 cases of meningiomas of different grades and 10 cases of normal brain tissue with ElivisionTM plus two-step method.We detected CD34 to display the tumor microvessel.Tumour angiogenesis was assayed by the value of MVD.Results The high levels of HIF-lα expression were detected in 23 of 45 in the meningiomas,the positive rate was 51.1%.The positive cells of HIF-lα displayed that yellow and deep yellow were stained in cytoplasm of the tumor cells,distributed like particles and cluster.The value of MVD are respectively 10.34±2.78,17.25±2.69,20.43±5.20 and 30.79±5.64 in group of Normal Brain tissue,WHO I,WHO II and WHO III.HIF-lα and MVD are positive correlation each other.Conclusion Intensity of HIF-lα expression relates to the angiogenesisof meningioma,has the stimulation of meningioma angiogenesis,provide the theoretical and experimental foundation further for clinical anti-angiogenesis therapy of meningiomas.