Ultrasound-assisted extraction of blackberry seed oil and analysis of its fatty acids

Blackberry seed oil was extracted by ultrasound-assisted method from blackberry seed.The effects of extraction solvents,ratio of solid to liquid,time and power of ultrasound were discussed by single factor and orthogonal experiments,and the optimum parameters were obtained.In addition,the fatty acid components of the seed oil were analyzed by gas chromatography mass spectrometry(GC-MS).The results indicated that the optimum parameters were as follows:petroleum ether(30-60℃) as extraction solvent,ratio of solid mass to liquid volume 1∶10,extraction time 10 min,ultrasound power 400 W.Under the optimum conditions the oil yield was 17.06%.The fatty acid components of blackberry seed oil were linoleic acid(76.00%),linolenic acid(15.48%),palmitic acid(5.81%),stearic acid(2.70%).