Supercritical Carbon Dioxide Extraction and Fatty Acid Composition Analysis of Blackberry Seed Oil

Objective: To extract blackberry seed oil from blackberry seeds with supercritical carbon dioxide and determine its fatty acid composition.Methods: Operating parameters that influence the extraction of blackberry seed oil were optimized using an orthogonal array design.The fatty acid composition of blackberry seed oil was analyzed by gas chromatography-mass spectrometry(GC-MS).Results: The optimal extraction conditions were extraction temperature of 35 ℃,extraction pressure of 12 MPa,separation pressure of 12 MPa and separation temperature of 55 ℃.Under thess conditions,the yield of blackberry seed oil was up to 16.10%.The major fatty acids were palmic acid(5.38%),stearic acid(3.53%),oleic acid(13.70%),linoleic acid(60.48%),gamma-linolenic acid(11.16%) and total unsaturated fatty acid(85.34%).