The Expression and Biological Significance of Skp2 in Tissue Microarray Including Lung Cancer and Precancerous Lesion

Objective In order to study the expressions of Skp2 in tissue microarray including lung cancer and precancerous lesion and the relationships between its expressions and clinicopathological parameters in primary lung cancer. With these results,we hope to be helpful for genesis,progress,gene therapy and prognosis evaluation of lung cancer. Methods We used SP immunohistochemistry method to detect the expressions of Skp2 in tissue microarray including lung cancer and precancerous lesion.All data were processed by SPSS 11.5 analysis soft ware. Results (1) Skp2 proteins were respectively detected in 0(0/10),16.7%(2/12),67.4%(60/89)and 83.3%(10/12)of normal lung tissue,precancerous lesion,primary lung cancer and lymph node metastasis of lung cancer; the positive rate of Skp2 protein in primary lung cancer and lymph node metastasis of lung cancer were significantly higher than that of normal lung tissue and precancerous lesion (P0.05),but the positive rate of Skp2 protein had no significant difference between normal lung tissue and precancerous lesion or between primary lung cancer and lymph node metastasis of lung cancer (P0.05). (2)In primary lung cancer,the positive rate of Skp2 protein was significantly higher in the group with lymph node metastasis than that without lymph node metastasis (P0.05); it was higher in the group of Ⅲ+Ⅳ stage than that inⅠ+Ⅱ stage (P0.05);the expression degrees of Skp2 protein had significantly positive correlation with the clinical stages of lung cancer (P0.05). But there were not significant difference between the groups of different histological types,gross types,ages,sexes and differentiation degrees (P0.05). Conclusion (1) The positive rate of Skp2 protein in primary lung cancer and lymph node metastasis of lung cancer are significantly higher than that of normal lung tissue and precancerous lesion,which suggests that Skp2 promote the genesis and progress of lung cancer; Skp2 may become a new target for gene therapy. (2) In primary lung cancer,the positive rate of Skp2 protein is significantly higher in the group with lymph node metastasis than that without lymph node metastasis,and it was higher in the group of Ⅲ+Ⅳ stage than inⅠ+Ⅱ stage. These suggests that Skp2 can promote the progress of lung cancer; Skp2 is related to the prognosis of lung cancer,which can be a reference in prognosis evaluation.