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2006•Journal of Pharmaceutical and Biomedical SciencesRequires access

Cloning and Identification of Sunflower Seed-specific Promoter Ha ds10 G1

Luo Yun-bo

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Abstract

The 1 414 bp 5' flanking sequence of Ha ds10 G1 gene, a late embryogenesis abundant gene of Helianthus annuus L., was cloned by PCR. The similarity compared with the reported sequence was 100%. The promoter was fused to the glucuronidase gene to construct plant expression vector, which was transferred into tobacco(Nicotiana tabacum) NC89 by Agrobacterium tumefaciens-mediated method. PCR results showed that the promoter had been integrated into genomic DNA of tobacco (Nicotiana tabacum) successfully. GUS activity assays indicated that expression of GUS was active only in transgenic tobacco seeds. However, the GUS activity didn't exist in the stems and leaves. So, the 1 414 bp 5' flanking sequence of Ha ds10 G1 gene is seed-specific promoter.

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What this paper is about

The 1 414 bp 5' flanking sequence of Ha ds10 G1 gene, a late embryogenesis abundant gene of Helianthus annuus L., was cloned by PCR. The similarity compared with the reported sequence was 100%. The promoter was fused to the glucuronidase gene to construct plant expression vector, which was transferred into tobacco(Nicotiana tabacum) NC89 by Agrobacterium tumefaciens-mediated method. PCR results showed that the promoter had been integrated into genomic DNA of tobacco (Nicotiana tabacum) successfully. GUS activity assays indicated that expression of GUS was active only in transgenic tobacco seeds. However, the GUS activity didn't exist in the stems and leaves. So, the 1 414 bp 5' flanking sequence of Ha ds10 G1 gene is seed-specific promoter.

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Available abstract

The 1 414 bp 5' flanking sequence of Ha ds10 G1 gene, a late embryogenesis abundant gene of Helianthus annuus L., was cloned by PCR. The similarity compared with the reported sequence was 100%. The promoter was fused to the glucuronidase gene to construct plant expression vector, which was transferred into tobacco(Nicotiana tabacum) NC89 by Agrobacterium tumefaciens-mediated method. PCR results showed that the promoter had been integrated into genomic DNA of tobacco (Nicotiana tabacum) successfully. GUS activity assays indicated that expression of GUS was active only in transgenic tobacco seeds. However, the GUS activity didn't exist in the stems and leaves. So, the 1 414 bp 5' flanking sequence of Ha ds10 G1 gene is seed-specific promoter.

Key concepts: Nicotiana tabacum, Biology, Agrobacterium tumefaciens, Gene, Cloning (programming), Sunflower, Transgene, Helianthus annuus

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