Propagation of PTP1B Gene Knockout Mice and Its Genotype Identification

[Objective] The research aimed to discuss the propagation and identification methods of PTP1B gene knockout mice and lay the foundation for further study on the function of protein tyrosine phosphatase.[Method] The introduced heterozygous mice were fed and propagated.After successful propagation,there were three genotypes(wild-type,heterozygote and homozygote) in the offspring.The genomic DNA was extracted from the tail tissue of each genotype mice and the wild type and knockout-gene fragment were amplified by using PCR method.And the genotypes of mice were judged according to the length of gene fragment.[Result] The intercrossing results of heterozygous mice with PTP1B gene were basically accordant with Mendelian inheritance laws and PTP1B gene-loss homozygous mice had no propagation ability.[Conclusion] The genotype of PTP1B gene mutant mice could be accurately identified by using PCR method.