Reproduction and Genotype Identification of Smad 3 Gene Knockout Mice

Objective To further study the important role of Smad3 gene during the vertebrate development, and reproduction of Smad3 gene knockout mice. Methods The heterozygous mice were used for breeding. The genotypes of the offspring were characterized using PCR and Southern blot. Both homozygous and wild type mice were used for phenotype analysis, and the heterozygous mice were used for propagating. Results The genotypes of 278 offsprings were characterized using PCR. 83 mice are wild type, 133 are heterozygotes, 62 are homozygotes. Conclusion The targeted Samd3 mutant gene could be inherited stably. The ratio of 3 genotypes of the offspring fits the Mendel′s laws.