Inhibitory effect of resveratrol on high glucose-induced proliferation of mesangial cells

Objective To evaluate the effect of resveratrol on glucose-induced proliferation of mesangial cells.Methods The cultured mesangial cells were divided into five groups:control,high glucose,2.5(2.5 μmmol/L resveratrol plus 25 mmol/L glucose),5(5 μmmol/L resveratrol plus 25 mmol/L glucose),and 10 μmmol/L group(10μmmol/L resveratrol plus 25 mmol/L glucose).The effect of resveratrol on mesangial cell proliferation was assessed with cell counting kit-8(CCK8).The percent of cell cycle and apoptosis were examined with flow-cytometry.Results The viability in high glucose group was 105.74±1.78% and significantly higher compared to that of the control group(P0.01).After treatment with resveratrol,the viability were 101.38±3.62%,96.55±1.28%,and 91.95±2.40%,which were lower compared to high glucose group(P0.05,P0.001).The percent of G1 in high glucose group increased compared to that of the control group(62.86%±1.57% vs 57.93%±2.70%,P0.05).After treatment with resveratrol,the percent of G1 in 10μmmol/L group decreased compared to that of the high glucose group(57.66%±2.49% vs 62.86%±1.57%,P0.05).The percent of S in high group reduced compared to that of the control group(29.00%±1.08% vs 34.91%±1.43%,P0.01).After treatment with resveratrol,the percent of S in 5 and 10 μmmol/L groups were increased compared to that of the high glucose group(P0.01).The percent of apoptosis in 5 and 10bμmmol/L groups were 2.20%±0.53% and 4.53±0.31%,and higher than that of the high glucose group(P0.01).Conclusion High glucose-induced proliferation of mesangial cells is inhibited by resveratrol therapy.The mechanisms of the inhibition include mesangial cell S phase arrest and cell apotopsis.