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2009•Journal of Anhui Traditional Chinese Medical CollegeRequires access

A Study on Apoptosis in Prostate Cancer Cells Line PC-3 Induced by Procyanidins

Shang Xuejun

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Abstract

Objective: To observe the effect of procyanidins on apoptosis in cultured prostate carcinoma PC-3 cells and its correlation with mitochondrial membrane potential(Δψm) changes.Methods: After treated with procyanidins 100,200,300 μg/ml respectively,PC-3 cells were doubly stained by Annexin Ⅴ-fluoresein isothiocyanate(FITC) and propidium iodide(PI).The procyanidins-induced apoptosis and necrosis were detected by flow cytometry(FCM).The change of Δψm was analyzed with FCM after Rhodamine 123 staining.Results: After treated with procyanidins 100 μg/ml for 24 hours,the apoptosis rate of PC-3 cells was 5.64%,and Δψm was significantly decreased in 84.7% of PC-3 cells.The apoptosis rate and necrosis rate of PC-3 cells was 44.86%,50.81% in the group treated with procyanidins 300 μg/ml,respectively.Conclusion: Procyanidins could induce apoptosis and necrosis in PC-3 cells,which might relate with down-regulation of mitochondrial Δψm.

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Objective: To observe the effect of procyanidins on apoptosis in cultured prostate carcinoma PC-3 cells and its correlation with mitochondrial membrane potential(Δψm) changes.Methods: After treated with procyanidins 100,200,300 μg/ml respectively,PC-3 cells were doubly stained by Annexin Ⅴ-fluoresein isothiocyanate(FITC) and propidium iodide(PI).The procyanidins-induced apoptosis and necrosis were detected by flow cytometry(FCM).The change of Δψm was analyzed with FCM after Rhodamine 123 staining.Results: After treated with procyanidins 100 μg/ml for 24 hours,the apoptosis rate of PC-3 cells was 5.64%,and Δψm was significantly decreased in 84.7% of PC-3 cells.The apoptosis rate and necrosis rate of PC-3 cells was 44.86%,50.81% in the group treated with procyanidins 300 μg/ml,respectively.Conclusion: Procyanidins could induce apoptosis and necrosis in PC-3 cells,which might relate with down-regulation of mitochondrial Δψm.

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Available abstract

Objective: To observe the effect of procyanidins on apoptosis in cultured prostate carcinoma PC-3 cells and its correlation with mitochondrial membrane potential(Δψm) changes.Methods: After treated with procyanidins 100,200,300 μg/ml respectively,PC-3 cells were doubly stained by Annexin Ⅴ-fluoresein isothiocyanate(FITC) and propidium iodide(PI).The procyanidins-induced apoptosis and necrosis were detected by flow cytometry(FCM).The change of Δψm was analyzed with FCM after Rhodamine 123 staining.Results: After treated with procyanidins 100 μg/ml for 24 hours,the apoptosis rate of PC-3 cells was 5.64%,and Δψm was significantly decreased in 84.7% of PC-3 cells.The apoptosis rate and necrosis rate of PC-3 cells was 44.86%,50.81% in the group treated with procyanidins 300 μg/ml,respectively.Conclusion: Procyanidins could induce apoptosis and necrosis in PC-3 cells,which might relate with down-regulation of mitochondrial Δψm.

Key concepts: Apoptosis, Propidium iodide, Annexin, Isothiocyanate, Rhodamine 123, Necrosis, Flow cytometry, Chemistry

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