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Simultaneous determination of hespefidin and baicalin in Qingqi Huatan Pill by HPLC

MA Li-xing

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Abstract

AIM: To establish a method for the simultaneous determination of hesperidin and baicalin in Qingqi Huatan Pill (Pericarpium Citri Reticulatae, Radix Scutellariae, etc.). METHODS: RP-HPLC was adopted with a mobile phase of acetonitrile-water-1% phosphoric acid (20∶80∶0.2), flow rate of 0.8 mL·min -1. The detection wavelength was at 280 nm. RESULTS:The linear range of hesperidin was 0.04 μg-0.37 μg, the average recovery was 97.5% and RSD was 1.0%. The linear range of baicalin was 0.13 μg-1.19 μg. The average recovery was 97.5%and RSD was 1.2%, respectively. CONCLUSION: This method is simple, practical, accurate and suitable for the quality control of Qingqi Huatan Pill.

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What this paper is about

AIM: To establish a method for the simultaneous determination of hesperidin and baicalin in Qingqi Huatan Pill (Pericarpium Citri Reticulatae, Radix Scutellariae, etc.). METHODS: RP-HPLC was adopted with a mobile phase of acetonitrile-water-1% phosphoric acid (20∶80∶0.2), flow rate of 0.8 mL·min -1. The detection wavelength was at 280 nm. RESULTS:The linear range of hesperidin was 0.04 μg-0.37 μg, the average recovery was 97.5% and RSD was 1.0%. The linear range of baicalin was 0.13 μg-1.19 μg. The average recovery was 97.5%and RSD was 1.2%, respectively. CONCLUSION: This method is simple, practical, accurate and suitable for the quality control of Qingqi Huatan Pill.

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Available abstract

AIM: To establish a method for the simultaneous determination of hesperidin and baicalin in Qingqi Huatan Pill (Pericarpium Citri Reticulatae, Radix Scutellariae, etc.). METHODS: RP-HPLC was adopted with a mobile phase of acetonitrile-water-1% phosphoric acid (20∶80∶0.2), flow rate of 0.8 mL·min -1. The detection wavelength was at 280 nm. RESULTS:The linear range of hesperidin was 0.04 μg-0.37 μg, the average recovery was 97.5% and RSD was 1.0%. The linear range of baicalin was 0.13 μg-1.19 μg. The average recovery was 97.5%and RSD was 1.2%, respectively. CONCLUSION: This method is simple, practical, accurate and suitable for the quality control of Qingqi Huatan Pill.

Key concepts: Baicalin, Hesperidin, Chromatography, Chemistry, Naringin, High-performance liquid chromatography, Radix (gastropod), Phosphoric acid

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