Protective effects of resveratrol on hippocampus neurons of subacute aging mice

Objective To investigate the protective effect of resveratrol(RES) on neurons in hippocampus in subacute aging mouse.Methods The subacute aging mice were prepared by subcutaneous injection of D-galactose at the nape.From the third week,the mice were fed with resveratrol(15.45 mg / kg) by oral gavage and the control group were fed with normal saline or solution of DMSO.The superoxide dismutase(SOD) activity and malondialdehyde(MDA) level in serum and brain of mice were detected.Morphology of hippocampal CA1 area was observed through Nissil stain.The expression of P53 protein was detected by immunohistochemistry.TUNEL was used to detect apoptosis.Results Morphology of hippocampal CA1 area had no change in all groups.Compared with the control groups,SOD activity was significantly increased and MDA content was significantly decreased in serum and brain of the mice fed with 45mg/kg resveratrol.The number of P53 positive cells was less in hippocampus CA1 zone of the mice fed with 45mg/kg resveratrol than that of mices in control group.Typical apoptotic cells can not be seen in hippocampus CA1 zone of all the groups.Conclusions Resveratrol can enhance the antioxidant function of subacute aging mouse,and decrese P53 expression in hippocampal CA1 neurons in subacute aging mouse.