Determination of ten β-agonists in feeds by high performance liquid chromatography-tandem mass spectrometry

A high performance liquid chromatography-tandem mass spectrometry method was established for the determination of ten β-agonists in feeds.β-agonists in sample were extracted by perchloric acid solution,purified and concentrated by HLB column.A C18 column was used to separate the β-agonists.Qualitative and quantitative analysis of ten β-agonists which ionized by electrospray ionization interface(ESI) was carried out by multiple reaction monitoring mode(MRM).The calibration curve was linear over the concentration range of 0.5～40.0 μg/L(r0.999).The recoveries ranged from 71.2% to 103% at the spiked level of 0.01,0.02,0.1,0.5 mg/kg.The relative standard deviations were between 3.7% and 9.8%.The limit of quantitation was 0.01 mg/kg.