Determination of Dexynivalenol in feed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry

A method has been developed for the determination of Dexynivalenol in feed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.The samples were extracted by phosphate buffer(pH 7.0) and purified by C18 solid phase extraction,and then analyzed by the LC-MS/MS after redissolved by 5% methanol.The mass spectrometry was run with electrospray ion source and in positive ions multiple reaction monitoring mode for the quantitative and qualitative analysis.The chromatographic column was a Phenomenex Luna 5μC18(2),and the flowing phase was comprised with methanol-0.1% formic acid solution(30:70).Limit of detection(LOD) was 20 μg/kg,and the limit of quantitation(LOQ) was 50 μg/kg.The recoveries ranged from 75.4% to 93.2% in 50,250 and 500 μg/kg concentration level.The relative standard deviation ranged from 5.74% to 7.62%.