Cationic liposome mediated NT-3 gene to transfect mice spiral ganglion cells

Objective To observe the localized expression of NT-3-pEGFPC2 and the influence to the growth of spiral ganglion cells(SGCs) by cationic liposome with the gene-NT-3-pEGFPC2 transfecting mice arche-generation spiral ganglion cells.Methods The eukaryonic expression vector(NT-3-pEGFPC2) with the report gene-pEGFPC2 was constructed.The SGCs were cultured and purged in vitro and identified by neurofilament(NF200) monoclonal antibody immunochemical staining.The NT-3-pEGFPC2 was transiently transfected into SGCs by using lipofectamine as the vector.The expression of NT-3 after transfection and the influence to the number of SGCs were observed.Results The SGCs were successfully cultured and identified.The cell bodies and axons of SGCs with green fluorescence were observed by fluorescence microscope.The expression of NT-3 was checked by the Western blot.The decreasing number of SGCs in the group transfected with NT-3 was less than that in the control group after twelve days.Conclusion Cationic liposome can be a vector of NT-3 in gene therapy.The degradation of SGCs can be alleviated by gene transfection of NT-3 in vitro.