Expression of cationic liposome-mediated neurotropin-3 in Schwann cell

Objective To observe the expression of cationic liposome-transfected neurotrophin-3(NT-3) gene in Schwann cells(SC) and increase the ability of SC to secret NT-3.Methods Liposome-mediated NT-3 was transfected into SC by cationic liposome intervention and divided into experimental group and control group.NT-3 expression was detected in SC with the ELISA double antibody sandwich method 1,2,4,and 8 weeks after transfection.DNA of SC was identified by enzyme digestion.Purity of SC was detected with immunohistology S-100 staining.Structure of transfected NT-3 was observed under an electron microscope.Results The expression level of NT-3 in SC was significantly higher 2,4 and 8 weeks after transfection than before transfection(P0.05).Enzyme digestion showed that the DNA level in transfected NT-3 gene was consistent with that in NT-3 gene fragments.The purity of SC was(94.1±2.3)% and(95.8±2.1)%,respectively,before and after transfection(P0.05).Conclusion The NT-3 gene can transfect cultured SC and highly expressed in them.As receptor cells,SC can be easily obtained and massively cultured in vitro.