Culture and identification of endothelial cells from calf thoracic aorta in vitro

Objective: To explore the primary culture method of calf thoracic aorta endothelial cells (CTAECs), enhance the success rate of separating and culturing CTAECs in vitro, establish the model of vascular endothelial cells, and provide experimental method for research of ECs. Methods: Endothelial cells were scratched from the tunia intima of calf thoracic aorta with scalpel. The cells were collected and cultured in fluid with 20% South African fetal calf serum. Their procreation and generation were observed, the morphologic characteristics of ECs were observed with light microscopy and immunohistochemistry. Results: Enough ECs were obtained from improved scratching method. Ⅷ factor in the ECs showed positive reaction by immunohistochemistry.Conclusion: Improved scratching method is an effective method to gain ECs, and can successfully establish the model for research of ECs.