GC-MS Analysis of the Extracts by Steam Distillation and Ethanol Extraction from Citrus Grandis Leaves

To analyse the chemical constituent of the extracts from leaves of Exocarpium Citrus Grandis by different extraction methods. The compounds were extracted by steam distillation and ethanol separately,constituents were separated and identified by GC-MS, and were quantified by the accuracy of area percent. 58 components were separated, among which 18 were identified, the main components were β-Pinene(19.44 %),β-Myrcene(6.95 %), Limonene(10.49 %), γ-Terpinene(19.30 %) and Caryophyllene(20.46 %) by steam distillation. 80 components were separated, among which 43 were identified, the main components were fatty acids(23.27 %) and sterols(27.22 %), the main active ingredients were five cumarins(Herniarin, Ficusin,Bergapte, Ammidin and Aurapten), Naringenin, Supraene and Vitamin E in the alcohol extraction. 18 chemical components of the extracts were the same by the two extraction methods, but there were more than 25 components in the alcohol extraction.