Experimental study on intra,tumor injection of slow,released 5,FU in the treatment of pancreatic carcinoma

Objective To study the effect of intra,tumor injection of slow,released 5,FU on pancreatic carcinoma; and to explore its mechanism of action. Methods Human pancreatic carcinoma strain PC,3 cells were cultured and ino,culated subcutaneously under the armpits of 70 Athymic mice. Sixty mice with similar tumor size were chosen 4 weeks afer inoculation. They were divided into 5 groups according to various modes of treatments given: NS intravenous injection as the control group, 5,FU (10 mg/kg) intravenous injection group, basic medium injection group, intra,tumor injection of slow,released 5,FU (4 mg/kg) group and intra,tumor injection of slow,released 5,FU (1 mg/kg) group. Tumor size was measured before treatment and 14 days after treatment. Tumors were excised. Tumor cell cycle kinetics was analyzed by microscopy. The apoptotic index (AI) was detected by terminal,deoxynucleotide transferase mediated d,UTP nick end labeling (TUNEL) and expression of bcl,2 and Bax by immunohistochemistry. Results The growth rate was lower in the 2 intra,tumor injection of slow,released 5,FU groups (treatment groups) than in other groups (P0.05). The Mitotic Index were lower in the 2 treatment groups than in the other groups (P0.05). The extent of local inflammation and increase in thickness of blood vessel endothelium were more evident in the treatment groups than in the other groups (P0.05). AI was significantly higher in the treatment groups than in the other groups (P0.05). The expression of bcl,2 was markedly decreased but that of Bax markedly increased in the treatment groups than in the other groups (P0.05). Conclusions Intra,tumor injection of slow,released 5,FU can inhibit the growth of pancreatic carcinoma by inducing local inflammation and thickening of blood vessel endothelium, and up,regulating apoptosis of the pancreatic cancer cells.